CC..png    

Legal and postal addresses of the publisher: office 1336, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation, Northern (Arctic) Federal University named after M.V. Lomonosov

Phone: (818-2) 21-61-21
E-mail: vestnik_med@narfu.ru
https://vestnikmed.ru/en/

ABOUT JOURNAL

Organometric and Morphometric Changes in the Testes of Immature Rats After Deliberately Induced Immunosuppression. P. 63–71

Версия для печати

Section: Medical and biological sciences

UDC

611.6+59.084:615.37

Authors

Svetlana A. Kashchenko*, Aleksey A. Zakharov*
*Lugansk State Medical University (Lugansk, Ukraine)

Abstract

Reproductive health is one of the criteria for determining life quality in a country. The male reproductive system is one of the most sensitive body systems that responds to environmental pollution, which leads to the development of immunosuppression. A considerable role in the development of homeostatic disorders is played by various medical effects, including deliberately induced immunosuppression. Our aim was to study organometric parameters of testes in immature experimental animals after immunosuppression. We studied changes in the structure of testes in 30 immature rats after administration of cyclophosphamide at a dose of 1.5 mg/kg body weight for 10 days. The rats were obtained from the vivarium of Lugansk State Medical University, where they had been kept under standard conditions and adequately fed. The experiment was guided by the ethical standards applicable to work with experimental animals. The rats were taken out from the experiment after 1, 7, 15, 30 and 60 days following drug administration. We measured the absolute and relative weight of the organ and its linear dimensions. On histological microslides we determined the diameter and area of the convoluted seminiferous tubules, as well as the thickness and area of the epitheliospermatogenic layer. The obtained data were processed using the license program StatSoft Statistica v6.0; the statistical significance of the difference between the indices of the experimental and control groups was determined by the Student-Fisher’s test (p < 0.05). Significant differences of organometric and morphometric parameters from the control data in the later stages of observation after drug administration were established, indicating an active response of testes to exogenous impacts. The absence of significant changes in the early stages of the experiment may be associated with the body’s systemic reaction to exogenous impacts, typical for this age period, and immaturity of the testis as a reproductive organ.

Keywords

immature rat’s testis, organometric features of testes, morphometric features of testes, cyclophosphamide, deliberately induced immunosuppression
Download (pdf, 1.5MB )

References

  1. Bessalova E.Yu. Biometricheskie pokazateli semennikov krys pri parenteral’nom vvedenii spinnomozgovoy zhidkosti [Biometric Indexes of White Rats’ Testicles After Injection of Cerebrospinal Fluid]. Visnik problem biologïï i meditsini, 2011, no. 4, pp. 195–197.
  2. Kashchenko S.A., Erokhina V.V. Ul’tramikroskopicheskie izmeneniya parashchitovidnykh zhelez krys posle korrektsii tsiklofosfan-indutsirovannoy immunosupressii imunofanom [Ultramicroscopic Changes in Rat Parathyroid Glands After Correction of Cyclophosphamide-Induced Immunosuppression with Imunofan]. Ukraїns’kiy morfologіchniy al’manakh, 2014, vol. 12, no. 1, pp. 61–64.
  3. Khlyakina O.V. Vliyanie neblagopriyatnykh ekologo-fiziologicheskikh faktorov na reproduktivnoe zdorov’e muzhchin v aspekte sovremennogo podkhoda k probleme i profilaktike muzhskogo besplodiya [Modern Approach to Influence of Adverse Ecological and Physiological Factors on Men’s Reproductive Health]. Vestnik Tambovskogo universiteta. Ser.: Estestvennye i tekhnicheskie nauki, 2011, vol. 16, no. 1, pp. 356–359.
  4. Kashchenko S.A., Petizina O.N., Morozova E.N. Korrelyatsionnaya zavisimost’ mezhdu parametrami limfaticheskikh uzlov krys posle korrektsii immunodefitsitnogo sostoyaniya [Correlation Between the Parameters of Rat Lymph Nodes After Correction of Immunodeficiency]. Ukraїns’kiy morfologіchniy al’manakh, 2014, vol. 12, no. 3, pp. 34–37.
  5. Banda E. Use of Animals in Research. European Science Foundation Policy Briefing, 2000, no. 9, pp. 1–6.
  6. Inflammatory Disorders of the Nervous System: Pathogenesis, Immunology, and Clinical Management. Ed. by A. Minagar, J.S. Alexander. Humana Press, 2005.
  7. Thappa D.M. Clinical Pediatric Dermatology. Elsevier, 2009.
  8. Vecchio M., Bonerba B., Palmer S.C., Craig J.C., Ruospo M., Samuels J.A., Molony D.A., Schena F., Strippoli G.F.M. Immunosuppressive Agents for Treating IgA Nephropathy (Review). Cochrane Database Syst. Rev., 2015, no. 8. Art. no.: CD003965.
  9. Vyshinskaya E.A. Intraperitoneal’naya khimioterapiya – put’ povysheniya effektivnosti lecheniya raka yaichnikov [Intraperitoneal Chemotherapy Is a Way to Increase the Effectiveness of Ovarian Cancer Treatment]. Sbornik nauchnykh trudov molodykh uchenykh: konf. 25 marta 2015 g. [Collected Works of Young Scientists: Conf. 25 March 2015]. St. Petersburg, 2015, pp. 21–25.
  10. Lykov A.P., Bondarenko N.A., Poveshchenko O.V., Kabakov O.V., Rayter T.V., Kazakov O.V., Strunkin D.N., Poveshchenko A.F., Konenkov V.I. Effekt ekzogennoy DNK pri eksperimental’nom rake molochnoy zhelezy u krys linii Wistar na proliferativnyy potentsial kletok gemo- i limfopoeza [The Effect of Exogenous DNA in Experimental Breast Cancer in Wistar Rats at Proliferative Activity of Hemo- and Lymphopoietic Cells]. Fundamental’nye issledovaniya, 2014, no. 8-4, pp. 886–890.
  11. Ovsyannikov A.A., Bachinskiy R.O. Izuchenie osobennostey gonadotoksicheskogo deystviya nitrobenzola i metiltretbutilovogo efira v usloviyakh kholodovogo stressa [The Study of Gonadotoxic Effect of Nitrobenzene and Methyl Tert-Butyl Ether Under Cold Stress]. Uspekhi sovremennogo estestvoznaniya, 2011, no. 8, pp. 124–125.
  12. Yuan G., Dai S., Yin Z., Lu H., Jia R., Xu J., Song X., Li L., Shu Y., Zhao X. Toxicological Assessment of Combined Lead and Cadmium: Acute and Sub-Chronic Toxicity Study in Rats. Food Chem. Toxicol., 2014, vol. 65, pp. 260–268.
  13. Bessalova E.Yu., Pikalyuk V.S., Tkach V.V., Korolev V.A. Morfologicheskie i biokhimicheskie pokazateli reproduktivnoy sistemy samtsov pri parenteral’nom vvedenii tserebrospinal’noy zhidkosti v eksperimente [Morphological and Biochemical Parameters of the Male Reproductive System at Parenteral Administration of Cerebrospinal Fluid in Experiment]. Vesennie anatomicheskie chteniya: materialy nauch. konf., posvyashch. pamyati dots. N.G. Nazimovoy [Spring Anatomic Readings: Proc. Sci. Conf. Dedicated to Assoc. Prof. N.G. Nazimov]. Grodno, 30 May 2014. Grodno, 2014, pp. 17–22.
  14. Sayapina I.Yu., Ogorodnikova T.L. Kolichestvennaya otsenka funktsional’noy aktivnosti semennikov krys na etapakh adaptatsii k nizkim temperaturam [Quantitative Estimation of Rat Testis Functional Activity at the Stages of Adaptation to Low Temperatures]. Politematicheskiy setevoy elektronnyy nauchnyy zhurnal Kubanskogo gosudarstvennogo agrarnogo universiteta, 2013, no. 89. Available at: http://ej.kubagro.ru/2013/05/pdf/23.pdf (accessed 21 October 2015).
  15. Dudenkova N.A. Morfofunktsional’nye osobennosti reproduktivnoy sistemy pri svintsovoy intoksikatsii: dis. … kand. biol. nauk [Morphological and Functional Features of the Reproductive System at Lead Intoxication: Cand. Biol. Sci. Diss.]. Saransk, 2015. 138 p.
  16. C aneguim B.H., Cerri P.S., Spolidório L.C., Miraglia S.M., Sasso-Cerri E. Structural Alterations in the Seminiferous Tubules of Rats Treated with Immunosuppressor Tacrolimus. Reprod. Biol. Endocrinol., 2009, no. 7. Available at: https://rbej.biomedcentral.com/articles/10.1186/1477-7827-7-19 (accessed 30 June 2016).
  17. C hen Y., Zhang Z., Lin Y., Lin H., Li M., Nie P., Chen L., Qiu J., Lu Y., Chen L., Xu B., Lin W., Zhang J., Du H., Liang J., Zhang Z. Long-Term Impact of Immunosuppressants at Therapeutic Doses on Male Reproductive System in Unilateral Nephrectomized Rats: A Comparative Study. Biomed Res. Int., 2013. Art. ID 690382. Available at: http:// dx.doi.org/10.1155/2013/690382 (accessed 30 June 2016).



Make a Submission


INDEXED IN: 

DOAJ_logo-colour.png

Elibrary.ru

logotype.png

infobaseindex

Логотип.png




Лань

OTHER NArFU JOURNALS: 

Vestnik of NArFU.
Series "Humanitarian and Social Sciences"

Forest Journal 
Лесной журнал 

Arctic and North