UDC

612.89+612.63+612.17

Authors

Andrey N. Trukhin
Vyatka State Humanities University
26 Krasnoarmeyskaya St., Kirov, 610002, Russian Federation;
е-mail: trukhinandrey@rambler.ru
Aleksey A. Kunshin
Vyatka State Humanities University
26 Krasnoarmeyskaya St., Kirov, 610002, Russian Federation;
е-mail: tabyss@bk.ru

Abstract

This paper summarizes the available data in scientific literature (mainly obtained in the Laboratory of Muscle and Bioactive Substance Physiology, Vyatka State Humanities University) indicating the presence in human urine and serum of the endogenous blocker and endogenous sensitizer of M-cholinergic receptors (EBMChR and ESMChR) affecting in vitro the activation efficiency of M-cholinergic receptors (MChR) of uterine smooth muscles, blood vessels, trachea, and myocardium. Part 1 of the survey focuses on the history of the discovery of M-cholinolytic activity of animal blood serum and the emergence of the term EBMChR, as well as EBMChR phenomenology exhibited through experiments with longitudinal stripes of nonpregnant rat uterine horns, It is indicated that the manifestation of blood serum EBMChR activity depends on its dilution factor (it is best detected at 1:10, 1:50 and 1:100 dilutions), while the relative content of EBMChR in human blood depends on the age (being higher at the initial stages of ontogenesis) and presence of somatic diseases (being increased at coronary heart disease and, in some patients, at bronchial asthma, but reduced at stage 3 hypertension). EBMChR content does not depend on sex and in women on the stage of their reproductive process or such obstetric complications as uterine inertia, threatened premature delivery, and pre-eclampsia. Scientists have been searching for EBMChR analogs, lysophosphatidylcholine being especially noteworthy, and suggest considering it as EBMChR component. On the basis of the facts presented in both parts of our survey, we make a conclusion that EBMChR plays an important role in а healthy human and animal body, while its deficiency or excess can be directly linked to the formation of a number of somatic diseases. It is clear that, together with other components of the humoral arm of the autonomic nervous system, EBMChR determines the degree of parasympathetic effects on visceral organs.

Keywords

acetylcholine, M-cholinergic receptors, endogenous modulators of M-cholinergic receptors, endogenous modulators of alpha- and beta-adrenergic receptors, smooth muscles, coronary vessels, myocardium, pregnancy

References

1. Zvezdina N.D., Turpaev T.M. Kholinoliticheskie svoystva syvorotki krovi [Cholinolytic Properties of Blood Serum]. Fiziologicheskiy zhurnal SSSR im. I.M. Sechenova, 1970, vol. 56, no. 8, pp. 1136–1141.
2. Zvezdina N.D., Prokazova N.V., Vaver V.A., Bergelson L.D., Turpaev T.M. Effect of Lysolecithin and Lecithin of Blood Serum on the Sensitivity of Heart to Acetylcholine. Biochem. Pharmacol., 1978, vol. 27, no. 10, pp. 2793–2801.
   
3. Suslova I.V., Korotaeva A.A., Prokazova N.V. Izmenenie parametrov ravnovesnogo svyazyvaniya [3N]- khinuklidinilbenzilata na membranakh predserdiya krolika pod deystviem lizofosfatidilkholina [Changes in the Equilibrium Binding of 3-Quinuclidinyl Benzilate on Rabbit Atrial Membranes on Exposure to Lysophosphatidylcholine]. Doklady Akademii nauk, 1995, vol. 342, no. 2, pp. 273–276.
   
4. Prokazova N.V., Zvezdina N.D., Korotaeva A.L. Vliyanie lizofosfatidilkholina na peredachu transmembrannogo signala vnutr’ kletki. Obzor [Effect of Lysophosphatidylcholine on the Transfer of Transmembrane Signal into the Cell. Overview]. Biokhimiya, 1998, vol. 63, no. 1, pp. 38–46.
   
5. Tsirkin V.I., Dvoryanskiy S.A., Osokina A.A., Ponomareva I.A., Snigireva N.L. Sposobnost’ syvorotki krovi cheloveka ingibirovat’ sokratitel’nuyu reaktsiyu miometriya na atsetilkholin [The Ability of Human Serum to Inhibit Contractile Response of Myometrium to Acetylcholine]. Lekarstvennoe obozrenie (Kirov), 1996, no. 4, pp. 49–54.
   
6. Tsirkin V.I., Dvoryanskiy S.A. Sokratitel’naya deyatel’nost’ matki (mekhanizmy regulyatsii) [Uterine Activity (Regulation Mechanisms)]. Kirov, 1997. 270 p.
   
7. Tsirkin V.I., Nozdrachev A.D., Sazanova M.L., Dvoryanskiy S.A. Fiziologicheskie svoystva miotsitov arteriy i veny pupoviny cheloveka i vliyanie na nikh syvorotki pupovinnoy krovi [Human Umbilical Artery and Vein Myocyte Physiological Properties and Umbilical Blood Serum Influence on Them]. Doklady Akademii nauk, 2003, vol. 388, no. 3, pp. 426–429.
   
8. Sizova E.N., Tsirkin V.I., Kostyaev A.A. Vliyanie ozona na sokratitel’nuyu aktivnost’ i khemoreaktivnost’ prodol’noy muskulatury roga matki neberemennykh krys [Effect of Ozone Exposure on Contractile Activity and Chemoreactivity of Uterus Horns Longitudinal Muscles of Nonpregnant Rats]. Rossiyskiy fiziologicheskiy zhurnal im. I.M. Sechenova, 2003, vol. 89, no. 4, pp. 427–435.
   
9. Sizova E.N., Tsirkin V.I., Trukhin A.N. Nalichie v krovi i likvore cheloveka endogennykh modulyatorov M-kholinoretseptorov [The Presence of Endogenous Modulators of M-Cholinergic Receptors in the Human Blood and Cerebrospinal Fluid]. Vestnik Pomorskogo universiteta. Ser.: Fiziologicheskie i psikhologo-pedagogicheskie nauki, 2004, no. 2(6), pp. 22–31.
   
10. Tsirkin V.I., Trukhin A.N., Sizova E.N., Makarova I.A., Khlybova S.V., Dvoryanskiy S.A. Vliyanie endogennykh modulyatorov M-kholinoretseptorov na M-kholinoreaktivnost’ miometriya i miokarda i na variabel’nost’ serdechnogo ritma [The Effect of Endogenous Modulators of M-Cholinergic Receptors on M-Cholinergic Reactivity of Myometrium and Myocardium on Heart Rate Variability]. Kazanskiy meditsinskiy zhurnal, 2006, vol. 87, no. 5, pp. 381–383.
    
11. Sizova E.N., Tsirkin V.I. Fiziologicheskaya kharakteristika endogennykh modulyatorov β-adreno- i M-kholinoreaktivnosti [Physiological Characteristics of Endogenous Modulators of β-Adrenergic and M-Cholinergic Reactivity]. Kirov, 2006. 183 p.
    
12. Demina N.L., Tsirkin V.I., Tarlovskaya E.I., Kashin R.Yu. α- i β-adreno-, M-kholinomoduliruyushchaya i miotsit-stimuliruyushchaya aktivnost’ syvorotki krovi pri arterial’noy gipertenzii [Alpha- and Beta-Adreno-, M-Cholinomodulating and Myocyte-Stimulating Serum Activity in Arterial Hypertension]. Kardiovaskulyarnaya terapiya i profilaktika, 2008, no. 2, pp. 16–22.
    
13. Mal’chikova S.V., Sizova E.N., Tsirkin V.I., Gulyaeva S.F., Trukhin A.N. M-kholinoblokiruyushchaya aktivnost’ syvorotki krovi pri ostrom koronarnom intsidente i vliyanie na nee fizicheskikh trenirovok [M-Cholinoblocking Activity of Blood Serum in Acute Coronary Events and the Effect of Physical Training]. Rossiyskiy fiziologicheskiy zhurnal im. I.M. Sechenova, 2003, vol. 89, no. 5, pp. 556–563.
    
14. Mal’chikova S.V., Sizova E.N., Tsirkin V.I., Gulyaeva S.F., Trukhin A.N., Vedernikov V.A. Izmenenie β-adrenosensibiliziruyushchey i M-kholinoblokiruyushchey aktivnosti syvorotki krovi u pozhilykh lyudey pri ostrom koronarnom intsidente i pod vliyaniem fizicheskikh trenirovok [Changes in Beta-Adrenosensitizing and M-Cholinoblocking Activity of Blood Serum in Older Adults at Acute Coronary Event and Under the Influence of Physical Training]. Kardiovaskulyarnaya terapiya i profilaktika, 2003, vol. 2, no. 6, pp. 36–43.
    
15. Tsirkin V.I., Nozdrachev A.D., Sizova E.N., Mal’chikova S.V., Gulyaeva S.F. Izmenenie soderzhaniya v krovi endogennykh modulyatorov β-adreno- i M-kholinoreaktivnosti pod vliyaniem fizicheskikh trenirovok u lits, perenesshikh infarkt miokarda [Changes in Blood Levels of Endogenous Modulators of Beta-Adrenergic and M-Cholinergic Reactivity as a Result of Physical Training in Patients with the History of Myocardial Infarction]. Byulleten’ eksperimental’noy biologii i meditsiny, 2003, vol. 136, no. 7, pp. 18–22.
    
16. Chulkina E.A., Gulyaeva S.F., Tsirkin V.I., Kostyaev A.A. M-kholinoblokiruyushchaya aktivnost’ syvorotki krovi cheloveka v opytakh s kol’tsevymi segmentami koronarnoy arterii svin’i [M-Cholinoblocker Activity of Human Blood Serum in the Experiments with Porcine Coronary Artery Ring Segments]. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo, 2014, no. 2(1), pp. 133–139.
    
17. Tsirkin V.I., Kononova T.N., Sizova E.N., Popova I.V., Vakhrusheva A.S., Kostyaev A.A., Kunshin A.A., Penkina Yu.A. β-Adreno- i M-kholinomoduliruyushchaya aktivnost’ syvorotki krovi i mochi pri bronkhial’noy astme [Beta-Adrenergic and M-Cholinomodulating Activity of Blood and Urine Serum in Bronchial Asthma]. Vyatskiy meditsinskiy vestnik, 2006, no. 1, pp. 53–65.
    
18. Tsirkin V.I., Kononova T.N., Sizova E.N., Popova I.V., Vakhrusheva A.S. O vozmozhnoy roli endogennykh modulyatorov β-adreno- i M-kholinoreaktivnosti v patogeneze bronkhial’noy astmy [About the Possible Role of Endogenous Modulators of β-Adrenoreactivity and M-Cholinoreactivity in the Pathogenesis of Asthma]. Pul’monologiya, 2007, no. 5, pp. 46–50.
    
19. Tsirkin V.I., Kononova T.N., Sizova E.N., Popova I.V., Vakhrusheva A.S. Changes in the β-Adrenergic and M-Cholinergic Modulating Activities of the Blood Serum and Urine in Bronchial Asthma. Human Physiology, 2008, vol. 34, no. 3, pp. 384–387.
    
20. Demina N.L., Tsirkin V.I., Tarlovskaya E.I., Kashin R.Yu., Penkina Yu.A. Soderzhanie v syvorotke krovi cheloveka endogennykh modulyatorov a- и b-adreno i M-kholinoreaktivnosti pri arterial’noy gipertenzii [The Human Blood Serum Level of Endogenous Modulators of Alpha- and Beta-Adrenergic and M-Cholinergic Reactivity at Hypertension]. Vyatskiy meditsinskiy vestnik, 2008, no. 1, pp. 33–42.
    
21. Kunshin A.A., Nozdrachev A.D., Tsirkin V.I., Trukhina S.I., Dvoryanskiy S.A., Pomaskina T.V., Gulyaeva S.F., Kostyaev A.N. Vliyanie syvorotki krovi cheloveka na M-kholino- i al’fa- i beta-adrenoreaktivnost’ gladkikh myshts zheludka krysy [Influence of Human Blood Serum on M-Cholino- and alpha- and Beta-Adrenoreactivity of Rat Stomach Smooth Muscles]. Vestnik Sankt-Peterburgskogo universiteta. Ser. 3: Biologiya, 2007, no. 3, pp. 40–53.
    
22. Gulyaev P.V., Pomaskina T.V., Kunshin A.A., Chervotkina L.A., Gulyaeva S.F., Tsirkin V.I. K mekhanizmu deystviya sul’fatno-kal’tsievoy mineral’noy vody nizhne-ivkinskaya 2K v kompleksnoy terapii kislotozavisimykh zabolevaniy pishchevaritel’nogo trakta [Mechanism of Action of Nizhne-Ivkinskaya 2k Sulfate-Calcium Mineral Water in Combined Treatment of Acid-Dependent Gastrointestinal Diseases]. Terapevticheskiy arkhiv, 2008, vol. 80, no. 1, pp. 23–28.
    
23. Tsirkin V.I., Nozdrachev A.D., Sazanova M.L., Dvoryanskiy S.A., Khlybova S.V. Uteroaktivnye, β-adrenomoduliruyushchie i M-kholinomoduliruyushchie svoystva syvorotki pupovinnoy krovi cheloveka [Uteroactive, Beta-Adrenomodulatory and M-Cholinomodulatory Properties of Human Umbilical Blood Serum]. Doklady Akademii nauk, 2003, vol. 388, no. 5, pp. 704–707.
    
24. Tsirkin V.I., Trukhin A.N., Sizova E.N., Dvoryanskiy S.A., Makarova I.A. Vliyanie syvorotki krovi beremennykh zhenshchin na sokratimost’ i khemoreaktivnost’ miometriya krysy i miokarda lyagushki [The Effect of Blood Serum of Pregnant Women on the Contractility and Chemoreactivity of Rat Myometrium and Frog Myocardium]. Vyatskiy meditsinskiy vestnik, 2003, no. 4, pp. 85–92.
    
25. Sizova E.N., Tsirkin V.I., Tumanova T.V. Vliyanie pishchevykh aminokislot na sokratitel’nuyu sposobnost’, b-adreno- i M-kholinoreaktivnost’ gladkikh myshts krys [Influence of Alimentary Amino Acids on Contractility, Beta- Adrenergic and M-Cholinergic Reactivity of Smooth Мuscles]. Voprosy pitaniya, 2008, vol. 77, no. 5, pp. 26–32.
    
26. Sizova E.N., Tsirkin V.I., Tumanova T.V. Issledovanie vliyaniya 20 aminokislot na β2-adreno- i M1-kholinoreaktivnost’ miometriya krysy [Research into the Effect of 20 Amino Acids on Beta2-Adrenergic and M1-Cholinergic Reactivity of Rat Myometrium]. Vyatskiy meditsinskiy vestnik, 2003, no. 4, pp. 80–85.
    
27. Sizova E.N., Tsirkin V.I., Tumanova T.V., Sazanov A.V. Vliyanie ryada lekarstvennykh veshchestv na β-adreno- i M-kholinoreaktivnost’ miometriya krysy [The Impact of Some Drugs on Beta-Adrenergic and M-Cholinergic Reactivity of Rat Myometrium]. Vyatskiy meditsinskiy vestnik, 2004, no. 1, pp. 25–31.
    
28. Kunshin A.A., Tsirkin V.I., Prokazova N.V. Influence of lysophosphatidylcholine, phosphatidylcholine, and hen egg yolk on contractile effects of acetylcholine on smooth muscles of rat stomach. Bull. Exp. Biol. Med., 2007, vol. 143, no. 6, pp. 663–666.
    
29. Kunshin A.A., Tsirkin V.I., Prokazova N.V., Dvoryanskiy S.A., Trukhina S.I. M-kholinomoduliruyushchie svoystva syvorotki krovi, lizofosfatidilkholina i kurinogo zheltka v opytakh s gladkimi myshtsami zheludka krysy [M-Cholinomodutatory Properties of Blood Serum, Lysophosphatidylcholine and Chicken Egg Yolk in Experiments with Rat Stomach Smooth Muscles]. Vyatskiy meditsinskiy vestnik, 2007, no. 2–3, pp. 96–101.
    
30. Toropov A.L., Korotaeva K.N., Samodelkina E.O., Tsirkin V.I., Vyaznikov V.A. Vliyanie lizofosfatidilkholina na adreno- i M-kholinoreaktivnost’ gladkikh myshts i miokarda [Influence of Lysophosphatidylcholine on Adreno- and M-Cholinoreactivity of Smooth Muscle and Myocarduim]. Vestnik Novosibskogo gosudarstvennogo universiteta. Ser.: Biologiya, klinicheskaya meditsina, 2010, vol. 8, no. 3, pp. 18–26.
    
31. Toropov A.L., Korotaeva K.N., Samodelkina E.O., Tsirkin V.I., Vyaznikov V.A., Prokazova N.V. Vliyanie lizofosfatidilkholina, yaichnogo zheltka i gistidina na adreno- i M-kholinoreaktivnost’ myshts [Influence of Lysophosphatidylcholine, Egg Yolk and Histidine on Adreno- and M-Cholinoreactivity of Muscle]. Vyatskiy meditsinskiy vestnik, 2010, no. 1, pp. 69–75.
    
32. Korotaeva K.N., Tsirkin V.I. Vliyanie lizofosfatidilkholina i yaichnogo zheltka na amplitudu vyzvannykh sokrashcheniy miokarda krysy [The Effect of Lysophosphatidylcholine and Egg Yolk on the Amplitude of Induced Contractions in Rat Myocardium]. Vestnik Nizhegorodskogo gosudarstvennogo universiteta im. N.I. Lobachevskogo, 2011, no. 1, pp. 110–116.
    
33. Korotaeva K.N., Nozdrachev A.D., Tsirkin V.I. Vliyanie syvorotki neberemennykh zhenshchin i lizofosfatidilkholina na effektivnost’ aktivatsii M-kholinoretseptorov miokarda krysy [Influence of Nonpregnant Women Blood Serum and Lizophosphatidilholine on Efficiency of Activation of Rat Myocardium M-Cholinoreactivity]. Vestnik Sankt-Peterburgskogo universiteta. Ser. 3: Biologiya, 2011, no. 3, pp. 57–65.
    
34. Kunshin A.A., Kononova T.N., Tsirkin V.I. Kurinyy yaichnyy zheltok snizhaet M-kholinoreaktivnost’ gladkikh myshts zheludka i matki [Chicken Egg Yolk Reduces M-Cholinergic Reactivity of Stomach and Uterine Smooth Muscles]. Parenteral’noe i enteral’noe pitanie: tez. dokl. IX Mezhdunar. kongr. [Parenteral and Enteral Nutrition: Outline Reports 9th Int. Congr.]. Moscow, 2005.
    
35. Ushkalova V.N., Ionidis N.V., Kadochnikova G.D., Deeva Z.M. Kontrol’ perekisnogo okisleniya lipidov [Control of Lipid Peroxidation]. Novosibirsk, 1993. 182 p.
    
36. Tsirkin V.I., Nozdrachev A.D., Sizova E.N., Dvoryanskiy S.A., Sazanova M.L. Sistema endogennoy modulyatsii, reguliruyushchaya deyatel’nost’ perifericheskikh avtonomnykh nervnykh struktur [Endogenous Modulation System Regulating the Activity of Peripheral Autonomic Nervous Structures]. Doklady Akademii nauk, 2002, vol. 383, no. 5, pp. 698–701.
    
37. Sizova E.N., Nozdrachev A.D., Tsirkin V.I., Dvoryanskiy S.A., Sazanova M.L. Gipoteza o sisteme endogennoy modulyatsii deyatel’nosti perifericheskikh avtonomnykh nervnykh struktur [Hypothesis About the System of Endogenic Modulation of Activity of Peripheral Autonomic Nervous Structures]. Vestnik Sankt-Peterburgskogo universiteta. Ser. 3: Biologiya, 2004, no. 2, pp. 40–46.