UDC

577.2(045)

Authors

Marina V. Egorova
Mordovian State Pedagogical Institute named after M.E. Evseviev
11a Studencheskaya St., Saransk, 430007, Russian Federation;
e-mail: egorowa.marina@mail.ru
Olga S. Shubina
Mordovian State Pedagogical Institute named after M.E. Evseviev
11a Studencheskaya St., Saransk, 430007, Russian Federation;
e-mail: o.shubina@mail.ru

Abstract

The research was conducted on 10 outbred white adult male rats weighing 200–250 g, areas of their cerebellar cortex being the material. We utilized histological and morphometric research methods to study the organization of neurons in the molecular, Purkinje, and granular layers of the cerebellar cortex. The investigations were carried out using Axio Imager.M2 digital microscope with AxioVision SE64 Rel. 4.8.3 and ZEN 2011 image analysing software. Statistical processing was performed using Microsoft Excel (Office XP) and Statistica 7.0, including the determination of the arithmetic mean (x) and standard error (s_x). We found that the molecular layer is 320.3 ± 7.17 μm, Purkinje layer 47.1 ± 0.60 μm, and the granular layer 620.2 ± 29.66 μm thick. The results showed that neurons in the cerebellar cortex differ from each other in terms of morphometric characteristics. Thus, for example, the mean area of basket neuron somas is 199.9 ± 2.34 μm², and the mean cell volume is 1115.2 ± 0.10 μm³. The mean cell area of stellate neurons is 69.9 ± 0.89 μm², and the mean volume is 493.9 ± 0.09 μm³. The area and volume of Purkinje cells is 732.9 ± 12.95 μm² and 8190.1 ± 11.89 μm², respectively. Granule cells have an area of 73.7 ± 0.71 μm² and a volume of 483.7 ± 0.06 μm³. Thus, the research has allowed us to study the characteristics of neurons in the white rat cerebellar cortex and show at the microscopic level the morphological universality of the molecular, Purkinje, and granular layers of the cerebellar cortex in white male rats during postnatal ontogenesis.

Keywords

molecular layer, Purkinje layer, granular layer, basket neurons, stellate neurons, Purkinje cells, granule cells

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