Legal and postal addresses of the publisher: office 1410a, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation, Northern (Arctic) Federal University named after M.V. Lomonosov

Phone: (818-2) 21-61-21
E-mail: vestnik_med@narfu.ru
http://medbio.narfu.ru/en/

ABOUT JOURNAL

Cytogenetic Abnormalities Induced in vitro in Peripheral Blood Lymphocytes of Healthy People by Borrelia garinii Antigen. P. 77–84

Версия для печати

Section: Medical and biological sciences

UDC

616-097.1+576.312/.312.8:575.224.232:575.224.234:001.891.53:579.834.114

Authors

Al’bert G. Semenov*, Ekaterina N. Il’inskikh*, Nikolay N. Il’inskikh*
*Siberian State Medical University (Tomsk, Russian Federation)

Abstract

It is known that some viruses and bacteria can be classified as biological mutagens. This paper aimed to characterize the different types of cytogenetic abnormalities induced in vitro by inactivated whole body Borrelia garinii antigen in peripheral blood lymphocyte cultures of healthy people. Phytohemagglutininstimulated cultures of peripheral blood lymphocytes were obtained from 13 healthy subjects uninfected with Lyme borreliosis to perform a chromosome analysis and study karyopathological and mitotic abnormalities. Inactivated whole body Borrelia garinii antigen was added to half of these cellular cultures, while the rest of the cultures remained intact. The research found that addition of the borrelia antigen to the lymphocyte cultures of healthy people induced a significant increase (as compared with the intact cultures) in the frequency of: structural chromosomal aberrations, with chromatid breaks and single fragments being predominant; cells with numerical cytogenetic abnormalities, such as hypoploidy or polyploidy; cells with various types of mitotic abnormalities, including chromosome lagging in mitosis, multigroup metaphases and multipolar mitosis; and cells with pathological nuclear morphology (binucleated and micronucleated cells). The detected cytogenetic abnormalities are similar to the chromosomal aberrations in peripheral blood lymphocyte cultures of patients with acute Lyme borreliosis, which can indicate genome instability of these immunocompetent cells not only in vitro, but also in vivo.

Keywords

Lyme borreliosis, chromosomal aberrations, aneuploidy, polyploidy, micronuclei, whole body antigen, Borrelia garinii
Download (pdf, 1.7MB )

References

  1. Il’inskikh I.N., Novitskiy V.V., Il’inskikh E.N., Il’inskikh N.N., Tkachenko S.B. Infektsionnaya kariopatologiya [Infectious Cariopahology]. Tomsk, 2005. 196 p. 
  2. Jinadasa R.N., Bloom S.E., Weiss R.S., Duhamel G.E. Cytolethal Distending Toxin: A Conserved Bacterial Genotoxin That Blocks Cell Cycle Progression, Leading to Apoptosis of a Broad Range of Mammalian Cell Lineages. Microbiology, 2011, vol. 157, no. 7, pp. 1851–1875. DOI: 10.1099/mic.0.049536-0 
  3. Molochnyy V.P., Solodovnikova O.N. Oksid azota neytrofilov i perekisnoe okislenie lipidov v tserebrospinal’noy zhidkosti u detey, bol’nykh gnoynymi meningitami [Nitric Oxide and Free Radical Processes of Cerebrospinal Fluid in Children with Bacterial Meningitis]. Dal’nevostochnyy meditsinskiy zhurnal, 2013, no. 2, pp. 17–19. 
  4. Il’inskikh E.N., Il’inskikh I.N., Semenov A.G. Cytogenetic Aberrations in Peripheral Blood Mononuclear Cells in Acute Lyme Borreliosis Patients. Cytol. Genet., 2013, vol. 47, no. 1, pp. 44–52. 
  5. Pirogova N.P., Novitskiy V.V., Khlusova M.Yu., Voronkova O.V., Karpova M.R., Lukashova L.V. Tsitogeneticheskiy status i fenotipicheskie svoystva limfotsitov perifericheskoy krovi u bol’nykh iksodovym kleshchevym borreliozom [Cytogenetic Status and Phenotypic Properties of Peripheral Blood Lymphocytes in Patients with Lyme Borreliosis]. Byulleten’ sibirskoy meditsiny, 2005, no. 3, pp. 43–48. 
  6. Cruz A.R., Moore V.M., La Vake C.J., Eggers C.H., Salazar J.C., Radolf J.D. Phagocytosis of Borrelia burgdorferi, the Lyme Disease Spirochete, Potentiates Innate Immune Activation and Induces Apoptosis in Human Monocytes. Infect. Immun., 2008, vol. 76, no. 1, pp. 56–70. DOI: 10.1128/IAI.01039-07 
  7. Ma Y., Seiler K.P., Tai K.F., Yang L., Woods M., Weis J.J. Outer Surface Lipoproteins of Borrelia burgdorferi Stimulate Nitric Oxide Production by the Cytokine-Inducible Pathway. Infect. Immun., 1994, vol. 62, no. 9, pp. 3663– 3671. 
  8. Moorhead P.S., Nowell P.C., Mellman W.J., Battips D.M., Hungerford D.A. Chromosome Preparations of Leukocytes Cultured from Human Peripheral Blood. Exp. Cell Res., 1960, vol. 20, pp. 613–616. DOI: 10.1016/0014- 4827(60)90138-5 
  9. Perticarari S., Presani G., Prodan M., Granzotto M., Murgia R., Cinco M. Lymphocyte Apoptosis Co-Cultured with Borrelia burgdorferi. Microb. Pathog., 2003, vol. 35, no. 4, pp. 139–145. 
  10. Alov I.A. Morfologicheskie i prikladnye aspekty patologii mitoza [Morphological and Applied Aspects of Pathologic Mitosis]. Arkhiv patologii, 1975, vol. 37, no. 12, pp. 3–14. 
  11. Christodoulides A., Boyadjian A., Kelesidis T. Spirochetal Lipoproteins and Immune Evasion. Front. Immunol., 2017, vol. 8. Art. no. 364. DOI: 10.3389/fimmu.2017.00364 
  12. Wei X., Yin H. Covalent Modification of DNA by α, β-Unsaturated Aldehydes Derived from Lipid Peroxidation: Recent Progress and Challenges. Free Radic. Res., 2015, vol. 49, no. 7, pp. 905–917. DOI: 10.3109/10715762.2015.1040009 
  13. Xu B., Wang W., Guo H., Sun Z., Wei Z., Zhang X., Liu Z., Tischfield J.A., Gong Y., Shao C. Oxidative Stress Preferentially Induces a Subtype of Micronuclei and Mediates the Genomic Instability Caused by p53 Dysfunction. Mutat. Res., 2014, vol. 770, pp. 1–8. DOI: 10.1016/j.mrfmmm.2014.08.004 
  14. Holt D.J., Grainger D.W. Multinucleated Giant Cells from Fibroblast Cultures. Biomaterials, 2011, vol. 32, no. 16, pp. 3977–3987. DOI: 10.1016/j.biomaterials.2011.02.021 
  15. Sohn J.J., Schetter A.J., Yfantis H.G., Ridnour L.A., Horikawa I., Khan M.A., Robles A.I., Hussain S.P., Goto A., Bowman E.D., Hofseth L.J., Bartkova J., Bartek J., Wogan G.N., Wink D.A., Harris C.C. Macrophages, Nitric Oxide and MicroRNAs Are Associated with DNA Damage Response Pathway and Senescence in Inflammatory Bowel Disease. PLoS ONE, 2012, vol. 7, no. 9. Art. no. e44156. DOI: 10.1371/journal.pone.0044156 
  16. Ohshima S., Seyama A. Cellular Aging and Centrosome Aberrations. Ann. N.Y. Acad. Sci., 2010, vol. 1197, pp. 108–117. DOI: 10.1111/j.1749-6632.2009.05396.x