UDC

616-092.16:616-092.9+599.323.4

Authors

Aksin’ya S. Lipatova*, Azamat Kh. Kade*, Artem I. Trofimenko*
*Kuban State Medical University (Krasnodar, Russian Federation)

Abstract

The research aimed to study the effectiveness of tDCS-therapy in combined stress-induced hormonal and cytokine status disorders in untrained male rats with high initial levels of stress tolerance and endurance. Group 1 (control) consisted of 10 intact rats. Based on the results of the 1st forced swim test, rats with the swimming time exceeding 371 s were selected for groups 2 and 3. Group 2 (comparison) included 8 rats that did not receive tDCS-therapy. Group 3 (treatment group) consisted of 8 rats that were subjected to 5 sessions of tDCS-therapy. In groups 2 and 3, the 2nd forced swim test was carried out on the 7th day of the experiment and orthostatic stress modelling with subsequent blood sampling, on the 8th day. We determined the content of adrenaline, ACTH, corticosterone, IL-1β, IL-6 and IL-10 in the blood plasma. Based on the results of the 1st forced swim test, the swimming time in rats with high levels of stress tolerance and endurance exceeded 371 s. The 2nd forced swim test revealed a reduction in swimming time by 91.4 % in Group 2. Orthostatic stress in rats from Group 2 was accompanied by an increase in blood plasma levels of adrenaline by 224.2 %, ACTH by the factor of 5.7, corticosterone by 58 %, IL-1β by 129.6 %, IL-6 by the factor of 4.4, and IL-10 by 28.4 %, compared to the control. According to the results of the 2nd forced swim test, 5 sessions of tDCS-therapy in Group 3 increased the swimming time by 79.9 %. Orthostatic stress was accompanied by a rise in the levels of adrenaline by 144.5 %, ACTH by the factor of 6.3, corticosterone by 25 %, IL-1β by 38.1 %, IL-6 by 54.1 %, and IL-10 by 10.8 %, in relation to the comparison group. Thus, we conclude that tDCS-therapy prevents maladaptation under combined stress.

Keywords

tDCS, combined stress, maladaptation, cytokines, adrenaline, corticosterone, stress tolerance

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