CC..png    

Legal and postal addresses of the publisher: office 1336, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation, Northern (Arctic) Federal University named after M.V. Lomonosov

Phone: (818-2) 21-61-21
E-mail: vestnik_med@narfu.ru
https://vestnikmed.ru/en/

ABOUT JOURNAL

Functional Characteristics of Peripheral Blood Leukocytes in People Aged 36–59 Years at Activation of the Purinergic Signalling System. P. 272–279

Версия для печати

Section: Physiology

UDC

[612.014+612.112]:576.385

DOI

10.17238/issn2542-1298.2019.7.3.272

Authors

Evgeniya A. Sladkova*, Elena A. Shamray*, Aleksandra Yu. Tishchenko*, Marina Yu. Skorkina*
*Belgorod National Research University (Belgorod, Russian Federation)
Corresponding author: Evgeniya Sladkova, address: ul. Pobedy 85, Belgorod, 308015, Russian Federation; e-mail: sladkova@bsu.edu.ru

Abstract

It is of particular interest to study the role of purinergic signalling of blood cells in cases of disturbed physiological regeneration, such as inflammatory processes and ageing. For leukocytes to realize their immune responses properly, the functional state of their plasma membrane is important, its main characteristics being stiffness, surface potential and strength of intercellular adhesion. To activate the elements of the purinergic signalling system, we applied a model of mechanical stress in vitro. Erythrocyte functional properties were studied using the methods of atomic force microscopy, namely, tapping mode and force spectroscopy, as well as tests for leukocyte migration activity. At simulated mechanical cell deformation, the level of adenosine triphosphate (ATP) in the blood increased by the factor of 2.3 as compared with the control. The cell surface charge and granulocyte stiffness decreased. Lymphocyte stiffness increased, while their surface potential became more negative, compared to the control. Granulocyte migration activity increased significantly. Moreover, a greater strength of adhesion between erythrocytes and leukocytes was detected. Thus, the findings indicate that the ATP molecule and its derivatives can affect leukocyte functional properties through auto- and paracrine regulation. The obtained experimental data expand the existing knowledge about the mechanisms of intercellular interaction between granulocytes and lymphocytes in the peripheral vessels, which can be of use when correcting abnormal immune response at ageing.

Keywords

purinergic signalling system, blood cells, surface potential, Young’s modulus, adhesive strength, migration activity, ageing
Download (pdf, 2.6MB )

References

1. Burnstock G. Purinergic Signalling. Br. J. Pharmacol., 2006, vol. 147, suppl. 1, pp. S172–S181.
2. Burnstock G. Purinergic Signalling: Pathophysiology and Therapeutic Potential. Keio J. Med., 2013, vol. 62, no. 3, pp. 63–73.
3. Kovalenko S.S., Yusipovich A.I., Parshina E.Yu., Maksimov G.V. Role of Purinergic Receptors of Erythrocytes in the Regulation of Conformation and Oxygen- and NO-Transporting Capacity of Hemoglobin. Bull. Exp. Biol. Med., 2015, vol. 159, no. 2, pp. 213–216.
4. Wang L., Olivercrona G., Götberg M., Olsson M.L., Sörhede Winzell M., Erlinge D. ADP Acting on P2Y13 Receptors Is a Negative Feedback Pathway for ATP Release from Human Red Blood Cells. Circ. Res., 2005, vol. 96, no. 2, pp. 189–196.
5. Baroja-Mazo A., Barberà-Gremades H., Pelegrín P. The Participation of Plasma Membrane Hemichannels to Purinergic Signaling. Biochim. Biophys. Acta, 2013, vol. 1828, no. 1, pp. 79–93.
6. Montalbetti N., Leal Denis M.F., Pignataro O.P., Kobatake E., Lazarowski E.R., Schwarzbaum P.J. Homeostasis of Extracellular ATP in Human Erythrocytes. J. Biol. Chem., 2011, vol. 286, no. 44, pp. 38397–38407.
7. Oonishi T., Sakashita K., Uyesaka N. Regulation of Red Blood Cell Filterability by Ca2+ Influx and cAMPMediated Signaling Pathways. Am. J. Physiol., 1997, vol. 273, no. 6, pp. C1828–C1834.
8. Sladkova E.A., Skorkina M.Yu. Estimation of Surface Potential of Lymphocytes from Patients with Leukemia Using Kelvin Probe Mode. Biophysics, 2014, vol. 59, no. 2, pp. 254–256.
9. Skorkina M.Yu., Fedorova M.Z., Zabinyakov N.A., Sladkova E.A. Sposob opredeleniya uprugosti kletok krovi [Method for Determining Blood Cell Elasticity]. Patent RF no. 2466401, 2011.
10. Skorkina M.Yu., Fedorova M.Z., Murav’ev A.V., Sladkova E.A. Ispol’zovanie nanomekhanicheskogo sensora dlya izucheniya morfofunktsional’nykh svoystv limfotsitov zdorovykh donorov i bol’nykh khronicheskim limfoblastnym leykozom [The Use of a Nanomechanical Sensor to Study the Morphofunctional Properties of Lymphocytes from Healthy Donors and Patients with Chronic Lymphocytic Leukemia]. Kletochnye tekhnologii v biologii i meditsine, 2012, no. 3, pp. 172–175.
11. Skorkina M.Yu., Shamray E.A., Sladkova E.A. Izmerenie sil adgezii v sisteme “kletka–kletka” na osnove tekhnologiy atomno-silovoy mikroskopii [Measuring of Adhesion Force in the Cell–Cell System Based on Atomic Force Microscopy Technology]. Kletochnye tekhnologii v biologii i meditsine, 2017, no. 4, pp. 213–215.
12. Novikov D.K., Novikov P.D., Titova N.D. Immunokorrektsiya, immunoprofilaktika, immunoreabilitatsiya [Immunocorrection, Immunoprophylaxis, Immunorehabilitation]. Vitebsk, 2006. 198 p.
13. Ellsworth M., Ellis C.G., Goldman D., Stephenson A.H., Dietrich H.H., Sprague R.S. Erythrocytes: Oxygen Sensor and Modulators of Vascular Tone. Physiology (Bethesda), 2009, vol. 24, pp. 107–116.
14. Olivieri A., Pala M., Gandini F., Hooshiar Kashani B., Perego U.A., Woodward S.R., Grugni V., Battaglia V., Semino O., Achilli A., Richards M.B., Torroni A. Mitogenomes from Two Uncommon Haplogroups Mark Late Glacial/ Postglacial Expansions from the Near East and Neolithic Dispersals Within Europe. PLoS One, 2013, vol. 8, no. 7. Art. no. e70492.
15. Li J., Fountain S.J. Fluvastatin Suppresses Native and Recombinant Human P2X4 Receptor Function. Purinergic Signal., 2012, vol. 8, no. 2, pp. 311–316.
16. De Ita M., Vargas M.H., Carbajal V., Ortiz-Quentero B., López-López C., Miranda-Morales M., Barajas-López C., Montaño L.М. ATP Releases ATP or Other Nucleotides from Human Peripheral Blood Leukocytes Through Purinergic P2 Receptors. Life Sci., 2016, vol. 145, pp. 85–92.
17. Junger W.G. Purinergic Regulation of Neutrophil Chemotaxis. Cell. Mol. Life Sci., 2008, vol. 65, no. 16, pp. 2528–2540.
18. North R.A. P2X Receptors. Philos. Trans. R. Soc. Lond. B Biol. Sci., 2016, vol. 371, no. 1700. Art. no. 20150427.
19. Goldman N., Chandler-Militello D., Langevin H.M., Nedergaard M., Takano T. Purine Receptor Mediated Actin Cytoskeleton Remodeling of Human Fibroblasts. Cell Calcium, 2013, vol. 53, no. 4, pp. 297–301.
20. Chen Y., Yao Y., Sumi Y., Li A., To U.K., Elkhal A., Inoue Y., Woehrle T., Zhang Q., Hauser C., Junger W.G. Purinergic Signaling: A Fundamental Mechanism in Neutrophil Activation. Sci. Signal., 2010, vol. 3, no. 125, pp. ra45.
21. Yip L., Cheung C.W., Corriden R., Chen Y., Insel P.A., Junger W.G. Hypertonic Stress Regulates T-Cell Function by the Opposing Actions of Extracellular Adenosine Triphosphate and Adenosine. Shock, 2007, vol. 27, no. 3, pp. 242–250.



Make a Submission


INDEXED IN: 

DOAJ_logo-colour.png

Elibrary.ru

logotype.png

infobaseindex

Логотип.png




Лань

OTHER NArFU JOURNALS: 

Vestnik of NArFU.
Series "Humanitarian and Social Sciences"

Forest Journal 
Лесной журнал 

Arctic and North