CC..png    

Legal and postal addresses of the publisher: office 1336, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation, Northern (Arctic) Federal University named after M.V. Lomonosov

Phone: (818-2) 21-61-21
E-mail: vestnik_med@narfu.ru
https://vestnikmed.ru/en/

ABOUT JOURNAL

Interrelation of Red Blood Cell and Platelet Levels and Haematocrit with Human Immune Response to Short-Term Whole-Body Cooling. P. 427–435

Версия для печати

Section: Physiology

UDC

612.111:612.017.1:612.014.43

DOI

10.17238/issn2542-1298.2019.7.4.427

Authors

Anna V. Samodova* ORCID: 0000-0001-9835-8083
Liliya K. Dobrodeeva* ORCID: 0000-0001-5080-6502
*N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences (Arkhangelsk, Russian Federation)
Corresponding author: Anna Samodova, address: prosp. Lomonosova 249, Arkhangelsk, 163061, Russian Federation; e-mail: annapoletaeva2008@yandex.ru

Abstract

This research involved 108 healthy subjects living in the city of Arkhangelsk and aged from 21 to 50 years (69 women and 39 men) before and after staying in a climate chamber for 5 minutes at –25 ºC. Using the haematological analyser XS-500i Sysmex (Japan) we studied red blood cell and platelet parameters as well as haematocrit levels in the peripheral venous blood. Erythrocyte aggregation was determined by microscopy of diluted blood according to the method of V.A. Levtov, S.A. Regirer and I.Kh. Shadrina (1982). We studied haemograms, neutrograms, monocytograms and lymphocytograms in blood film stains according to the Romanovsky-Giemsa method, as well as the phagocytic activity of neutrophils, and lymphocyte phenotypes (CD3+, CD4+, CD8+, CD10+, CD19+, CD23+, CD71+, CD95+) using the method of indirect immunoperoxidase reaction. The levels of IL-1β, TNF-α and IL-6 cytokines, irisin, and endothelin-1 were determined by the enzyme-linked immunosorbent assay. The majority of the examined individuals (70.37 %) showed no differences in haematocrit levels, erythrocyte content or haemoglobin before and after short-term cooling. In 23.15 % of the subjects, after the climate chamber the haematocrit level increased concurrently with the growing erythrocyte and haemoglobin levels; in 6.48 % of cases, the proportion of blood volume occupied by erythrocytes decreased. The rise in haematocrit levels is associated with increasing platelet concentration. In 20 % of cases, after the climate chamber the examined individuals showed an increase in red blood cell aggregation and in the average size of erythrocyte aggregates with a concurrent decrease in the share of non-aggregated erythrocytes. It was established that an increase in blood viscosity at cooling is combined with a noticeable decrease in the content of all types of lymphocytes and their phenotypes due to redistribution of myeloid cells from the circulating pool to the marginal pool with the participation of endothelin-1 vasoconstrictor.

Keywords

haematocrit, erythrocyte aggregation, platelets, neutrophils, lymphocytes, endothelin-1, irisin, cold exposure
Download (pdf, 0.7MB )

References

1. Kim L.B. Vliyanie polyarnogo stazha na kislorodotransportnuyu funktsiyu krovi u severyan razlichnogo vozrasta [Influence of Polar Time Record on the Oxygen Transportation Function of Blood of Northerners of Various Age]. Arktika i Sever, 2014, no. 17, pp. 150–162.
2. Mchedleshvili G.I. Gemoreologiya v sisteme mikrotsirkulyatsii: ee spetsifika i prakticheskoe znachenie (obzor) [Haemorheology in the Microcirculation System: Its Specificity and Practical Significance (Review)]. Tromboz, gemostaz i reologiya, 2002, no. 4, pp. 18–24.
3. Murav’ev A.V., Cheporov S.V. Gemoreologiya (eksperimental’nye i klinicheskie aspekty reologii krovi) [Haemorheology (Experimental and Clinical Aspects of Blood Rheology)]. Yaroslavl, 2009. 178 p.
4. De Lorenzo F., Kadziola Z., Mukherjee M., Saba N., Kakkar V.V. Haemodynamic Responses and Changes of Haemostatic Risk Factors in Cold-Adapted Humans. QJM, 1999, vol. 92, no. 9, pp. 509–513.
5. Stoltz J.E., Donner M. New Trends in Clinical Hemorheology: An Introduction to the Concept of the Hemorheological Profile. Schweiz. Med. Wochenschr., 1991, vol. 43, pp. 41–49.
6. Meerson F.Z., Minyaylenko T.D., Pozharov V.P., Seredenko M.M. Narusheniya vneshnego dykhaniya, transporta i utilizatsii kisloroda pri stresse [Disturbed External Respiration, Oxygen Transport and Utilization in Stress]. Patologicheskaya fiziologiya i eksperimental’naya terapiya, 1989, no. 6, pp. 20–26.
7. Gorizontova M.P. Mikrotsirkulyatsiya pri stresse [Microcirculation in Stress]. Patologicheskaya fiziologiya i eksperimental’naya terapiya, 1986, no. 3, pp. 79–85.
8. Levtov V.A., Regirer S.A., Shadrina N.Kh. Reologiya krovi [Blood Rheology]. Moscow, 1982. 270 p.
9. Shapovalov K.G., Sizonenko V.A., Vitkovskiy Yu.A., Mikhaylichenko M.I., Tomina E.A., Kovalev V.V. Izmeneniya adgezivnoy funktsii limfotsitov k trombotsitam pri mestnoy kholodovoy travme [Changing in Adhesive Function of Lymphocytes to Platelets at Local Cold Trauma]. Byulleten’ VSNTs SO RAMN, 2007, no. 5, pp. 182–183.
10. Lichtman M.A., Beutler E., Kipps Т.J., Seligsohn U., Kaushansky K., Prchal J.Т. Williams Hematology. New York, 2006. 2297 р.
11. Sokolova I.A. Agregatsiya eritrotsitov [Erythrocyte Aggregation]. Regionarnoe krovoobrashchenie i mikrotsirkulyatsiya, 2010, vol. 9, no. 4, pp. 4–26.
12. Proskurin A.I., Petrov V.V., Levitan B.N., Prokhvatilova Yu.I., Dedov S.V., Shirokov N.A. Gemoreologiya i mozgovoy krovotok u bol’nykh khronicheskimi gnoynymi sinuitami pri travmakh golovy [Haemorheology and Cerebral Blood Flow in Patients with Chronic Purulent Sinusitis at Head Injuries]. Uspekhi sovremennogo estestvoznaniya, 2004, no. 12, pp. 24–28.
13. Burduli N.M., Gutnova S.K. Vliyanie razlichnykh metodov nizkointensivnoy lazernoy terapii na agregatsionnye svoystva eritrotsitov pri khronicheskom pankreatite [Effect of Various Methods of Low-Intensity Laser Therapy on the Aggregation Properties of Red Blood Cells in Chronic Pancreatitis]. Eksperimental’naya i klinicheskaya gastroenterologiya, 2009, no. 7, pp. 9–13.
14. Dunaev A.V. Sudebno-meditsinskaya otsenka gistologicheskikh izmeneniy miokarda predserdiy v sluchae vnezapnoy smerti ot ostroy ishemicheskoy bolezni serdtsa [Forensic Evaluation of Histological Changes in the Myocardium of the Atria in Sudden Death from Acute Coronary Heart Disease]. Morfologiya, 2008, vol. 2, no. 4, pp. 15–19. DOI: 10.26641/1997-9665.2008.4.15-19
15. Vikhriev B.S., Kichemasov S.Kh., Skvortsov Yu.R. Mestnye porazheniya kholodom [Local Cold Lesions]. Leningrad, 1991. 192 p.
16. Buckton K.E., Brown W.M.C., Smith P.G. Lymphocyte Survival in Men Treated with X-Rays for Ankylosing Spondylitis. Nature, 1967, vol. 214, no. 5087, pp. 470–473.



Make a Submission


INDEXED IN: 

DOAJ_logo-colour.png

Elibrary.ru

logotype.png

infobaseindex

Логотип.png




Лань

OTHER NArFU JOURNALS: 

Vestnik of NArFU.
Series "Humanitarian and Social Sciences"

Forest Journal 
Лесной журнал 

Arctic and North